USMLE Vault · Divine Intervention OB/GYN
Step 2 / 3 · Study Guide
OB/GYN System

OB/GYN

5 episodes · 3 Rapid Review inline · Divine Intervention Podcast

High-yield obstetrics and gynecology for USMLE Step 2/3 — teratogens, fetal heart rate tracings, hypertensive disorders of pregnancy, postpartum hemorrhage, amenorrhea workup, contraception, AIS vs Müllerian agenesis, and cervical cancer screening, extracted from Divine Intervention and organized for exam performance.

1 episode
Normal Pregnancy & Prenatal Care
Teratogen questions are among the highest-yield OB/GYN topics across all three Steps — they test pharmacology, physiology, and clinical judgment simultaneously. The key is knowing not just which drugs are dangerous, but why: mechanism-driven recall lets you extrapolate to novel vignettes even when the specific drug name is unfamiliar.
EP350
The Clutch Teratogen Podcast
  • Isotretinoin (Accutane): Disrupts HOX genes → limb placement anomalies, craniofacial defects. Requires two forms of contraception + iPLEDGE registration before prescribing
  • Warfarin: Crosses placenta → fetal hemorrhage, stippling of epiphyseal plates, CNS defects. Use heparin or LMWH in pregnancy (do not cross placenta)
  • ACE inhibitors / ARBs: Renal agenesis → fetal anuria → oligohydramnios (amniotic fluid index <5 cm) → Potter sequence (limb deformities, pulmonary hypoplasia, abnormal facies)
  • Fetal alcohol syndrome: Smooth/hypoplastic philtrum + thin upper lip + short palpebral fissures + microcephaly. VSD is the most common cardiac defect. #1 preventable cause of intellectual disability in the US
  • Valproic acid: Neural tube defects (never use in pregnancy — even if stopping it causes seizures, the rule stands); also hepatotoxic. Safe AED in pregnancy: lamotrigine
  • Cocaine: Sympathomimetic vasoconstriction of placental vessels → placental abruption (painful bright-red third-trimester bleeding) + asymmetric IUGR (head spared, body small)
TeratogensWarfarinIsotretinoinFetal Alcohol SyndromeACE InhibitorsValproic AcidIUGR

Master Teratogen Table

Drug / AgentMechanismFetal / Neonatal EffectHY Integration
IsotretinoinHOX gene disruptionCraniofacial, limb, cardiac defectsMust have 2 contraceptives + iPLEDGE; stop before pregnancy
WarfarinVitamin K antagonist — crosses placentaFetal hemorrhage, epiphyseal stippling, CNS defectsSwitch to heparin/LMWH in pregnancy (prosthetic valves: heparin bridge)
ACE inhibitors / ARBsBlock renal development → oliguriaRenal agenesis, oligohydramnios, Potter sequenceClue: AFI <5 cm in woman on lisinopril/losartan
Statins (HMG-CoA inhibitors)Block cholesterol synthesis necessary for fetal developmentMultiple congenital anomaliesHold statins in all pregnant women, even with high CV risk
MethotrexateDihydrofolate reductase inhibitor → blocks DNA synthesisNeural tube defects, abortifacientUsed to treat ectopic pregnancy — stop before trying to conceive
Alcohol (FAS)Neuronal apoptosis, midline disruptionSmooth philtrum, thin lip, short palpebral fissures, microcephaly, VSDPhiltrum abnormality on exam = FAS until proven otherwise
Valproic acidFolate antagonismNeural tube defects (most severe of AEDs)NEVER use in pregnancy, regardless of seizure control
DanazolPro-androgenicVirilization of female fetus, precocious puberty in malesUsed for endometriosis — stop before pregnancy
ThalidomideAnti-angiogenicPhocomelia (proximal limb absent; distal extremities attach to shoulder/hip)Rare in modern practice but classic NBME vignette
AminoglycosidesOtotoxic — ablates CN8Sensorineural hearing loss → language delayNewborn doesn't turn toward sound or respond = audiometry
TetracyclinesBind calcium in bones and teethTooth discoloration, bone growth suppressionAvoid <8 years old. Exception: Rocky Mountain Spotted Fever at ANY age (including pregnancy)
LithiumCardiac teratogen (mechanism unclear)Ebstein's anomaly (downward displacement of tricuspid valve, "atrialization" of RV)Bipolar disorder in pregnancy: switch to antipsychotic
Paroxetine (SSRI)Serotonin disruption in pulmonary vasculaturePersistent pulmonary hypertension of newbornOnly SSRI contraindicated in pregnancy; sertraline is safest SSRI
CocaineVasoconstriction of placental arteriesPlacental abruption, asymmetric IUGR (head normal, body small)Painful third-trimester bleeding in IV drug user = abruption
DES (diethylstilbestrol)Hormonal disruption of Müllerian developmentClear cell adenocarcinoma of vagina/cervix, T-shaped uterus, recurrent pregnancy lossVaginal adenocarcinoma (not SCC) in young woman = DES exposure
CyclophosphamideAlkylating agent — cross-links DNALimb defects, cleft palate, neural tube defectsUsed for GPA (Wegener's) — must stop before/during pregnancy
ChloramphenicolImpairs mitochondrial 70S ribosomes → overwhelms neonatal glucuronidationGray baby syndrome: ashen color, cardiovascular collapseCommon in developing world; immigrant mother vignette
TMP-SMX / PyrimethamineFolate synthesis inhibitorsNeural tube defectsToxoplasmosis in pregnancy: use spiramycin (not pyrimethamine/sulfadiazine)
ErgotamineVasoconstrictive + uterotonicIUGR, premature labor, fetal demiseMigraine med — avoid in pregnancy; safe chronic prophylaxis = propranolol, topiramate
Gestational diabetes (IDM)Fetal hyperinsulinemia from maternal hyperglycemiaVSD, TGA, caudal regression syndrome (sirenomelia), neonatal RDS (insulin inhibits surfactant), hypoglycemia/hypocalcemia seizures, small left colonTerm baby with RDS = think gestational DM, not prematurity
Absolute Rules for Pregnancy

Never use: Valproic acid (any seizure severity), warfarin (switch to heparin), ACE inhibitors / ARBs, statins, methotrexate, isotretinoin (also requires 2 contraceptives), live attenuated vaccines (varicella, MMR, intranasal influenza — except rotavirus in infants under 1 year).

Give to all newborns of HBsAg-positive mothers: HBIg + HBV vaccine immediately at delivery (passive + active immunization).

HIV-positive mother's newborn: Give zidovudine (AZT) prophylaxis.

Symmetric vs Asymmetric IUGR

TypeHead vs BodyCausesTiming
Symmetric IUGRHead and body both smallTORCH infections, chromosomal abnormalities (trisomies), early teratogen exposureEarly pregnancy (first trimester)
Asymmetric IUGRHead normal, body small (brain-sparing)Placental insufficiency, cocaine, late teratogen exposureLate pregnancy (second/third trimester)
Polyhydramnios vs Oligohydramnios

Polyhydramnios (too much fluid): Fetus cannot swallow — anencephaly, tracheoesophageal fistula, duodenal atresia ("double bubble"), neuromuscular disorders. AFI >24 cm.

Oligohydramnios (too little fluid): Fetus cannot urinate — renal agenesis (Potter sequence), bilateral renal agenesis, ACE inhibitor exposure. AFI <5 cm. Leads to pulmonary hypoplasia + limb deformities from uterine wall compression.

Safe Drugs in Pregnancy (High-Yield)

  • Antihypertensives: Labetalol (first-line), hydralazine, nifedipine, methyldopa. Avoid ACE/ARB. Avoid atenolol (less fetal bradycardia data than labetalol)
  • Antiepileptics: Lamotrigine is preferred. If patient is seizure-controlled on phenytoin and stable, do not abruptly discontinue (risk of status epilepticus > teratogen risk) — except valproic acid, which must be stopped regardless
  • Antibiotics: Penicillins, cephalosporins, azithromycin, clindamycin, nitrofurantoin (avoid third trimester). Avoid tetracyclines, fluoroquinolones, aminoglycosides, TMP-SMX
  • Antidepressants: Sertraline (safest SSRI). Avoid paroxetine
  • Anticoagulation: Heparin (unfractionated or LMWH) — does not cross placenta. Warfarin and DOACs are contraindicated
3 episodes + Rapid Review
Obstetric Complications
Fetal heart rate tracings, hypertensive disorders of pregnancy, and postpartum hemorrhage form the core of inpatient OB management on the USMLE. The key to FHR tracings is the VEAL-CHOP mnemonic plus understanding the mechanism behind each pattern — exam questions frequently ask for the first management step, not just the diagnosis. Preeclampsia management hinges on knowing specific BP thresholds and delivery indications.
EP338
Fetal Heart Rate Tracings Made Easy
  • Normal FHR: 110–160 bpm. Tachycardia (>160) = maternal fever, fetal anemia, maternal hypotension, fetal hypoxia. Bradycardia (<110) = maternal beta-blocker use, neonatal lupus (anti-Ro/La antibodies → complete heart block)
  • Reactive non-stress test: At least 2 accelerations (>15 bpm above baseline for ≥15 seconds) within 20 minutes
  • Variable decelerations: Umbilical cord compression. Abrupt drop/return (<30 sec nadir). First step: left lateral decubitus position. If persistent: amnioinfusion or reduce oxytocin
  • Early decelerations: Head compression → Cushing's reflex bradycardia. Nadir coincides with contraction peak. Gradual (>30 sec). Benign — no intervention needed
  • Late decelerations: Uteroplacental insufficiency. Nadir comes AFTER contraction peak. Gradual (>30 sec). First step: left lateral decubitus. Repetitive late decels → urgent delivery (C-section)
  • Sinusoidal pattern: Sine-wave FHR = severe fetal anemia (Rh isoimmunization, fetal-maternal hemorrhage)
Fetal Heart RateVEAL-CHOPVariable DecelerationsLate DecelerationsNon-Stress TestUmbilical Cord

VEAL-CHOP Mnemonic

FHR FindingCauseMechanismManagement
Variable decelerationsCord compressionUmbilical cord compressed → ↑ SVR → baroreceptor → parasympathetic → ↓ HR. Abrupt: nadir in <30 secondsLeft lateral decubitus (first) → amnioinfusion (second) → reduce oxytocin
Early decelerationsHead compression↑ ICP from head compression → Cushing's reflex → vagal bradycardia. Gradual: nadir in >30 seconds, coincides with contraction peakNone — benign, physiologic
AccelerationsOkayFetal movement → sympathetic response → ↑ HR. Sign of fetal well-beingNone — reassuring
Late decelerationsPlacental insufficiencyUteroplacental blood flow inadequate → fetal hypoxia. Gradual: nadir in >30 seconds, comes AFTER contraction peakLeft lateral decubitus → supplemental O₂ → stop oxytocin → if repetitive: urgent delivery
Key Differentiator: Early vs Late Decelerations

Early: nadir = same time as contraction peak. Think: mirror image of contraction (symmetrical). Benign.
Late: nadir comes AFTER contraction peak. The delay = time it takes for placental insufficiency to register. Ominous — requires intervention.

Both are gradual (>30 sec from onset to nadir). Variable decelerations are abrupt (<30 sec).

Causes of Fetal Tachycardia vs Bradycardia

Fetal Tachycardia (>160)Fetal Bradycardia (<110)
Maternal fever (↑ metabolic rate)Maternal beta-blocker (labetalol for preeclampsia)
Fetal anemia (↑ cardiac output to compensate)Neonatal lupus (anti-Ro/anti-La → complete heart block)
Maternal hypotension (fetal compensatory ↑ HR)Prolonged cord compression (late-stage variable decel)
Fetal hypoxia (compensatory early; becomes bradycardia if severe)Fetal hypothyroidism (rare)

Sinusoidal Pattern

A sine-wave FHR pattern (smooth, regular undulations, no variability, no accelerations) indicates severe fetal anemia. Causes: Rh isoimmunization (anti-D antibodies), massive fetal-maternal hemorrhage, fetal hydrops, severe fetal infection. Management: urgent delivery or intrauterine transfusion.

First Step in ANY Abnormal FHR = Left Lateral Decubitus

For both variable and late decelerations, the first management step on NBME exams is left lateral decubitus position. This relieves aortocaval compression (IVC compression by the gravid uterus), increasing maternal cardiac preload → increased uteroplacental perfusion. If the question doesn't list this, then the next answer is amnioinfusion (for variable decels) or delivery (for repetitive late decels).

RR 70EP369
Hypertensive Disorders of Pregnancy, Eclampsia & Gestational Complications
  • Gestational hypertension: BP ≥140/90 after 20 weeks, no proteinuria, no end-organ damage. Normalizes by 12 weeks postpartum
  • Preeclampsia: BP ≥140/90 after 20 weeks + proteinuria (≥300 mg/24h or protein:Cr ≥0.3) OR end-organ damage (thrombocytopenia, renal insufficiency, elevated liver enzymes, pulmonary edema, new-onset headache/visual changes)
  • Severe features: BP ≥160/110, thrombocytopenia <100K, creatinine >1.1, transaminases 2× ULN, pulmonary edema, headache/visual symptoms. Deliver if ≥34 weeks; if <34 weeks consider steroids + magnesium then deliver
  • HELLP syndrome: Hemolysis (↑ LDH, schistocytes) + Elevated Liver enzymes + Low Platelets. Definitive treatment: delivery (can occur before 20 weeks or postpartum)
  • Eclampsia: Preeclampsia + seizures. Treatment: IV magnesium sulfate (first-line anticonvulsant) + antihypertensives. Delivery is curative
  • Safe antihypertensives in pregnancy: Labetalol (IV acute), hydralazine (IV acute), nifedipine (oral). Never ACE inhibitors or ARBs
PreeclampsiaEclampsiaHELLPMagnesiumGestational HTNLabetalol

Hypertensive Disorders of Pregnancy — Classification

ConditionBP ThresholdProteinuriaEnd-Organ DamageManagement
Chronic HTN≥140/90 before 20 weeks or pre-existingNo (unless superimposed)NoLabetalol or nifedipine; deliver at 37 weeks
Gestational HTN≥140/90 after 20 weeksNoNoMonitor; deliver at 37 weeks
Preeclampsia (without severe features)≥140/90 after 20 weeksYes (≥300 mg/24h)NoDeliver at 37 weeks; serial monitoring
Preeclampsia with severe features≥160/110 (or any BP with end-organ damage)Yes or No (end-organ enough)YesDeliver if ≥34 weeks; magnesium seizure prophylaxis; antihypertensives
HELLPVariableVariableYes (hemolysis, liver, platelets)Deliver regardless of gestational age; platelets if <50K before delivery
EclampsiaVariableVariableYes + seizuresIV magnesium (first-line), secure airway, antihypertensives, then deliver
Magnesium Toxicity — Know the Sequence

Magnesium is the treatment for eclamptic seizures AND prophylaxis in severe preeclampsia. Signs of toxicity in order:
1. Loss of patellar reflexes (first sign, 7–10 mEq/L) → 2. Respiratory depression (10–13 mEq/L) → 3. Cardiac arrest (>15 mEq/L).
Antidote: calcium gluconate IV (not calcium chloride — gluconate is safer peripherally).

HELLP Syndrome — Diagnosis

  • Hemolysis: ↑ LDH, ↑ indirect bilirubin, schistocytes on peripheral smear
  • ELevated Liver enzymes: AST/ALT >2× ULN
  • LP: Low Platelets <100,000/µL
  • Can occur without classic hypertension/proteinuria — diagnose on labs
  • Mimics: TTP, HUS, acute fatty liver of pregnancy (AFLP). AFLP = hypoglycemia + coagulopathy + liver failure

Placenta Previa vs Placental Abruption

Placenta PreviaPlacental Abruption
Bleeding characterPainless, bright redPainful, dark red; "woody" uterus
OnsetThird trimester (any time)Acute (third trimester)
CausePlacenta implanted over cervical osPremature placental separation; cocaine, HTN, trauma
Next stepTransabdominal ultrasound (NO digital exam — can precipitate massive hemorrhage)Clinical diagnosis + ultrasound; urgent delivery if severe
DeliveryC-section (placenta over os = cannot deliver vaginally)Vaginal delivery if stable; C-section if fetal distress or severe bleeding
DIC riskLowHigh (placental thromboplastin release)
Gestational Diabetes — Key Numbers

Screening: 1-hour 50g glucose challenge at 24–28 weeks (threshold: ≥140 mg/dL for abnormal → proceed to diagnostic test).
Diagnosis: 3-hour 100g OGTT. Abnormal if ≥2 values exceed: fasting 95, 1-hr 180, 2-hr 155, 3-hr 140 mg/dL.
Management: Diet first → insulin if diet fails. Metformin and glyburide are used but not FDA-approved for GDM.
Fetal complications: Macrosomia, shoulder dystocia, neonatal hypoglycemia, neonatal RDS (insulin inhibits surfactant), caudal regression syndrome, VSD/TGA.

RR 104EP487
Postpartum Hemorrhage — Causes, Algorithm, and Drug Choices
  • Definition: Blood loss >500 mL vaginal delivery or >1000 mL C-section. Most common cause: uterine atony (80%)
  • Uterine atony risk factors: Multiple gestation (twins/triplets), prolonged labor, polyhydramnios, chorioamnionitis, high parity. Uterus has "run out of energy" to contract
  • Management algorithm: Uterine massage (first, rarely an exam answer) → oxytocin (pitocin) → tranexamic acid (TXA) → misoprostol → carboprost → ergotamine → surgical (B-Lynch suture, uterine artery ligation, hysterectomy)
  • Carboprost (15-methyl PGF2α): Prostaglandin → uterine contraction. Contraindicated in asthma (bronchospastic). Alternative: misoprostol (PGE1) — safe in asthma
  • Tranexamic acid (TXA): Lysine analog → antifibrinolytic → prevents clot breakdown. Use early for hemorrhage
  • The 4 T's of PPH causes: Tone (atony), Trauma (lacerations), Tissue (retained placenta), Thrombin (coagulopathy)
Postpartum HemorrhageUterine AtonyOxytocinCarboprostTranexamic Acid4 Ts

PPH Management Algorithm

StepInterventionMechanismKey Contraindication
1stBimanual uterine massageMechanical compression; stimulates contractionNone (rarely an NBME answer — usually they ask what's next)
2ndOxytocin (Pitocin) IVUterotonic; compresses myometrial vessels to reduce bleedingNone in acute setting
3rdTranexamic acid (TXA)Antifibrinolytic (lysine analog); stabilizes clotHistory of thromboembolic disease (relative)
4thMisoprostol (PGE1)Prostaglandin → uterine contractionSafe in asthma
5thCarboprost (15-methyl PGF2α)Prostaglandin → uterine contraction (more potent)Asthma (bronchospastic — do NOT use)
6thMethylergonovine (ergot)Vasoconstrictive uterotonicHypertension, vascular disease, Raynaud's
SurgicalB-Lynch suture → uterine artery ligation → hysterectomyMechanical hemostasisIf all medical therapy fails
Oxytocin Mechanism — Why It Stops Bleeding

Blood vessels run between the myometrial muscle fibers. When oxytocin causes myometrial contraction, it mechanically compresses ("ligates") these intramyometrial vessels. This is analogous to surgical ligation — stopping the bleeding source without cutting. This is why uterine massage works too: same principle, mechanical.

4 T's of PPH

TCauseDiagnosisTreatment
ToneUterine atony (80%)Soft, boggy uterus on examUterotonic drugs (oxytocin, carboprost, misoprostol)
TraumaCervical/vaginal/uterine lacerationsFirm uterus but ongoing bleeding; inspect carefullySurgical repair
TissueRetained placenta or placental fragmentsIncomplete placenta at delivery, uterus won't contractManual extraction or uterine curettage
ThrombinCoagulopathy (DIC from abruption, AFLP, HELLP, amniotic fluid embolism)↑ PT/PTT, ↓ fibrinogen, schistocytesFFP, cryoprecipitate, platelets; treat underlying cause
Amniotic Fluid Embolism (AFE)

Sudden cardiovascular collapse + hypoxia + DIC during or immediately after labor/delivery. Fetal cells and amniotic fluid enter maternal circulation → complement activation → massive inflammatory cascade. Management: supportive (ICU, FFP, platelets, oxygen). Very high maternal mortality (>50%). Differentiate from PE (no DVT history, onset during delivery) and sepsis (no fever prodrome).

Incorporated in EP338
Labor & Delivery
The intrapartum period is where most obstetric emergencies occur. Fetal monitoring interpretation is paired here with C-section indications, operative vaginal delivery, and shoulder dystocia management — the practical application of the FHR principles from EP338 in the delivery room context.
EP338
Intrapartum Fetal Monitoring & Delivery Management
  • Category I FHR: Normal baseline (110–160), moderate variability, accelerations present, no late/variable decels. Routine monitoring only
  • Category III FHR (ominous): Sinusoidal pattern OR absent baseline variability with recurrent late decels, recurrent variable decels, or bradycardia → immediate delivery or intrauterine resuscitation then delivery
  • C-section indications: Category III FHR not responsive to resuscitation, placenta previa, prior classical (vertical) uterine incision, active HSV/HIV outbreak, cord prolapse, malpresentation (transverse lie)
  • Cord prolapse management: Elevate presenting part manually (take pressure off cord) → emergency C-section. Do NOT push cord back in
  • Shoulder dystocia: Head delivers but shoulders impacted behind pubic symphysis. First step: McRoberts maneuver (hyperflexion of maternal hips) + suprapubic pressure. If fails: Woods screw, Zavanelli, episiotomy
  • Oxytocin (Pitocin) use in labor: Augment or induce contractions. Excess → uterine hyperstimulation (tachysystole) → placental insufficiency → late decelerations. Treat by stopping oxytocin + left lateral decubitus
Labor ManagementC-Section IndicationsCord ProlapseShoulder DystociaMcRoberts ManeuverOxytocin

Stages of Labor

StageDefinitionSubstagesKey Facts
Stage 1Onset of labor → full dilation (10 cm)Latent (0–6 cm, slow), Active (6–10 cm, faster)Active phase: ≥1 cm/hr in nulliparous. Prolonged active phase = arrest → augment or C-section
Stage 2Full dilation → delivery of babyPushing phase. Prolonged (>3 hrs nulliparous, >2 hrs multiparous with epidural) → consider operative delivery
Stage 3Delivery of baby → delivery of placentaNormal: <30 min. >30 min = retained placenta
Stage 4First 2 hours postpartumHighest risk for PPH — monitor uterine tone and bleeding

Shoulder Dystocia Management (HELPERR)

  • Help — call for additional personnel immediately
  • Episiotomy — consider (provides room but does not solve the bony impaction)
  • Legs — McRoberts maneuver (hyperflex maternal thighs against abdomen → flattens lumbar lordosis → ↑ pelvic outlet)
  • Pressure — suprapubic pressure (NOT fundal pressure — makes it worse)
  • Enter — rotational maneuvers (Woods screw: push anterior shoulder toward fetal back; Rubin II: push anterior shoulder toward fetal chest)
  • Remove posterior arm — manually deliver posterior arm first
  • Roll — Gaskin (all-fours) position
What NOT to Do in Shoulder Dystocia

Never apply fundal pressure — drives shoulder further into the pubic symphysis and worsens impaction. Suprapubic pressure (downward + lateral) is correct. Never pull on the fetal head — risks brachial plexus injury (Erb's palsy: C5-C6, "waiter's tip" position).

Operative Vaginal Delivery

ForcepsVacuum
Fetal riskFacial nerve injury, cephalohematomaCephalohematoma, subgaleal hemorrhage, retinal hemorrhage
Maternal riskVaginal/perineal lacerations (3rd/4th degree)Same but less
IndicationProlonged second stage, fetal distressSame
ContraindicationFetal coagulopathy (hemophilia), <34 weeksFetal coagulopathy, <34 weeks (more fragile vessels)
3 episodes
Gynecologic Disorders
Amenorrhea workup, contraception counseling, and primary amenorrhea due to hormonal or anatomic causes are all high-frequency exam topics. The progestin/estrogen challenge test is a classic localization question — know which result points to anovulation vs hypoestrogen vs outflow obstruction. Contraception requires knowing specific contraindications by mechanism rather than memorizing lists.
EP373
Progestin and Estrogen Challenge Tests — Amenorrhea Workup
  • Test indication: Amenorrhea workup (rule out pregnancy first). Always do progestin challenge first, then estrogen-progestin if needed
  • Progestin challenge (positive = bleeds): Patient lacked progestin (did not ovulate → no corpus luteum → no progesterone). Diagnosis: anovulation (e.g., PCOS)
  • Progestin challenge (negative = does not bleed) → do estrogen-progestin challenge: If bleeds = patient was hypo-estrogenic. Causes: Turner syndrome (45,XO), Kallmann syndrome (no GnRH), hypothalamic dysfunction, premature ovarian insufficiency
  • Both challenges negative (no bleed): Outflow tract obstruction or uterine unresponsiveness. Diagnoses: Asherman syndrome (intrauterine adhesions from D&C), transverse vaginal septum, imperforate hymen
  • Asherman syndrome: Multiple D&C → intrauterine adhesions → endometrium cannot respond to hormones AND physical outflow obstruction. No bleed on either challenge
  • Kallmann syndrome: GnRH deficiency → low FSH/LH → low estrogen → primary amenorrhea + anosmia (inability to smell — olfactory bulb agenesis)
AmenorrheaProgestin ChallengeAnovulationPCOSAsherman SyndromeKallmann SyndromeTurner Syndrome

Challenge Test Decision Algorithm

Challenge PerformedResultInterpretationClassic Diagnosis
Progestin aloneBleeds (withdrawal bleed)Progesterone was the missing piece → no ovulation occurredAnovulation (PCOS, hypothyroidism, hyperprolactinemia)
Progestin aloneNo bleed → proceed to E+PNot just progesterone deficiency — something else is wrong
Estrogen + ProgestinBleedsEstrogen was deficient — once supplemented, endometrium respondedHypoestrogen state: Turner syndrome, Kallmann syndrome, premature ovarian insufficiency, hypothalamic amenorrhea
Estrogen + ProgestinNo bleedUterus/outflow cannot respond even with hormonesAsherman syndrome, imperforate hymen, transverse vaginal septum
Why the Progestin Challenge Works — The Physiology

The normal menstrual cycle: follicular phase (estrogen builds endometrium) → LH surge → ovulation → corpus luteum forms → progesterone (converts endometrium to secretory) → corpus luteum dies → progesterone falls → withdrawal bleed.

In anovulation (e.g., PCOS): estrogen is made but no ovulation → no corpus luteum → no progesterone. The endometrium is primed with estrogen but has no progesterone withdrawal trigger. When you give exogenous progestin and then withdraw it — you simulate the end of the luteal phase → withdrawal bleed occurs. This confirms the endometrium was primed (estrogen was present) and the only missing piece was progesterone (anovulation).

Primary Amenorrhea — Differential by Karyotype and Anatomy

ConditionKaryotypeGonadsUterusKey Feature
Turner syndrome45,XOStreak gonads (no estrogen)Present (small)Shield chest, webbed neck, bicuspid aortic valve, coarctation; ↑ FSH/LH; ↓ estrogen
Androgen Insensitivity (AIS)46,XYTestes (intra-abdominal)Absent (AMH destroys Müllerian ducts)Phenotypically female, no pubic/axillary hair (Tanner 1-2), breast development intact; ↑ testosterone
Müllerian Agenesis (MRKH)46,XXOvaries (normal)Absent (Müllerian ducts don't form)Normal breast and pubic hair (all Tanner 5); normal testosterone; vaginal canal absent or blind
Kallmann Syndrome46,XX or 46,XYNormal but non-functionalPresentAnosmia + primary amenorrhea; ↓ GnRH → ↓ FSH/LH → ↓ estrogen
Hypothalamic amenorrhea46,XXNormalPresentLow BMI, extreme exercise, stress; ↓ GnRH → low FSH/LH; reversible
AIS vs MRKH — The Critical Differentiator

Both: Phenotypically female, primary amenorrhea, absent uterus.
AIS: 46,XY, testes present (intra-abdominal, malignant potential → gonadectomy after puberty), breast development good, pubic/axillary hair sparse (androgens don't work).
MRKH: 46,XX, ovaries present, breast development good, pubic/axillary hair good (ovaries make estrogen and testosterone normally).
The exam question tells you: If Tanner stage for pubic hair is 1-2 but breasts are Tanner 5 = AIS (androgen receptor defect). If both breasts and pubic hair are Tanner 5 = MRKH (ovaries work fine).

Secondary Amenorrhea Causes (Mnemonic: PIRATE)

  • Pregnancy — always rule out first with β-hCG
  • Iatrogenic — oral contraceptives, Depo-Provera (post-pill amenorrhea)
  • Rax hypothyroidism / hyperprolactinemia — TSH and prolactin are first-line tests
  • Asherman syndrome — prior D&C; no bleed on both challenge tests
  • Tumors — prolactinoma (galactorrhea + amenorrhea), Cushing's
  • Exercise / Eating disorders — hypothalamic dysfunction (low GnRH)
EP531
The HY Contraceptive Podcast
  • First-line (no contraindications): LARC — Long-Acting Reversible Contraceptive. Includes IUDs (Mirena and copper) and Nexplanon (subdermal implant, 3 years). Most effective reversible methods
  • Combined OCP contraindications (absolute): History of VTE/stroke/MI, age >35 + smoker, migraines with aura, uncontrolled HTN, breast cancer, liver failure/hepatic adenomas, pregnancy/postpartum breastfeeding, immobilization, hypercoagulable states (Factor V Leiden, protein C/S deficiency)
  • Combined OCP benefits: Reduces ovarian cancer (↓ ovulations) + endometrial cancer. Treats acne, endometriosis, dysmenorrhea, PMS. Raises BP — biggest cause of HTN in reproductive-age women
  • Progestin-only (safe postpartum): Mini-pill, Mirena IUD, Nexplanon, Depo-Provera. No estrogen → safe for breastfeeding. Depo: delayed fertility return (up to 2 years), reversible ↓ bone density → calcium/vitamin D supplementation
  • Copper IUD: Non-hormonal LARC. Contraindicated: Wilson's disease, active STI within 6 months, distorted uterine anatomy, heavy bleeding/coagulopathy. Best emergency contraception (effective up to 7 days post-coitus)
  • CYP450 inducers ↓ OCP efficacy: Rifampin (TB), phenytoin, carbamazepine, phenobarbital, griseofulvin, primidone, St. John's Wort
ContraceptionLARCCombined OCPCopper IUDMirenaNexplanonEmergency ContraceptionCYP450

Contraceptive Methods — Organized by Type

MethodTypeDuration / NotesHY Feature
Nexplanon implantProgestin LARC3 years; subdermal inner armNo effect on bone density; rapid return to fertility after removal; LARC on USMLEs
Mirena IUDProgestin LARC5 yearsAlso treats adenomyosis (heavy bleeding + boggy uterus); rapid return to fertility; safe postpartum
Copper IUDNon-hormonal LARC10 years; emergency contraception ≤7 daysContraindicated: Wilson's disease; causes heavier periods; foreign body reaction destroys sperm
Depo-ProveraProgestin injectionEvery 3 monthsReversible ↓ bone density (supplement calcium/vit D); delayed fertility return (up to 2 years); not a LARC
Combined OCPEstrogen + progestin oralDaily; 21/7 or 28-day packProgestin thickens cervical mucus, inhibits LH surge; estrogen suppresses FSH/follicle development
NuvaRingCombined vaginal ring3 weeks in, 1 week outRisk: toxic shock syndrome (foreign body in vagina + high fever, desquamation, hypotension)
Transdermal patchCombined1 patch/week × 3 weeks, 1 week offSame contraindications as combined OCP
Progestin-only pillProgestin oralDaily at same timeMust take at SAME TIME every day (small progestin-only window); safe postpartum/breastfeeding
Levonorgestrel (Plan B)Emergency OCPWithin 72 hours (↓ efficacy by 72h); less effective than copper IUDNot effective after 72 hours; copper IUD superior up to 7 days
UlipristalEmergency (SPRM)Within 120 hoursProgesterone receptor modulator; more effective than levonorgestrel 72–120 hrs
Combined OCP — Absolute Contraindications

Hypercoagulability states: History of VTE, DVT, PE, stroke, MI — estrogen raises clotting risk. Factor V Leiden, protein C/S deficiency, antithrombin III deficiency (also lost in nephrotic syndrome), antiphospholipid syndrome.
Vascular disease: Age >35 + active smoking, uncontrolled hypertension, migraines with aura (already elevated stroke risk).
Hormone-sensitive cancers: Breast cancer (estrogen/progestin receptor positive).
Liver: Cirrhosis, active hepatitis, hepatic adenoma (estrogen stimulates adenoma growth).
Breastfeeding/postpartum: Estrogen reduces milk quantity and quality — use progestin-only methods instead.

OCP Benefits — Beyond Contraception

  • Reduces ovarian cancer risk (↓ lifetime ovulations → ↓ repetitive epithelial repair → ↓ cancer risk)
  • Reduces endometrial cancer risk (progestin suppresses endometrial proliferation)
  • Treats endometriosis (progestin suppresses ectopic endometrial tissue)
  • Treats PCOS (suppresses androgens → improves acne and hirsutism)
  • Reduces acne (consider as answer when OCP is asked in acne vignette)
  • Treats primary dysmenorrhea and PMS
  • Reduces menstrual blood loss (helpful in iron deficiency anemia)

CYP450 Inducers That Reduce OCP Efficacy

  • Rifampin (TB treatment — classic vignette: starts TB meds, becomes pregnant)
  • Phenytoin (epilepsy)
  • Carbamazepine (epilepsy, trigeminal neuralgia, bipolar)
  • Phenobarbital / Primidone (primidone used for restless leg syndrome — classic NBME vignette)
  • Griseofulvin (onychomycosis / ringworm)
  • St. John's Wort (depression supplement)

Complications: IUD Insertion

ComplicationPresentationManagement
Uterine perforationHeavy bleeding + lower abdominal pain + foreign body on ultrasound outside uterine cavityConservative if stable; laparoscopy if symptomatic
ExpulsionIUD strings longer than expected on exam or IUD seen at cervical osConfirm location with ultrasound; reinsert or choose alternate method
Infection / PIDFever, pelvic pain, cervical motion tenderness within 3 weeks of insertionAntibiotics; remove IUD if no improvement
Ectopic pregnancyIUD does not prevent implantation 100% — if pregnancy occurs, high ectopic riskMethotrexate (if stable) or salpingectomy
RR 100EP476
Androgen Insensitivity Syndrome & Müllerian Agenesis
  • AIS (androgen insensitivity syndrome): Also called testicular feminization. Karyotype 46,XY but phenotypically female. Testosterone receptor defective → testosterone has no effect → wolfian ducts don't virilize → phenotypically female
  • AIS presentation: Primary amenorrhea + Tanner stage 5 breasts + Tanner stage 1-2 pubic/axillary hair. No uterus (testes produce AMH → Müllerian duct destroyed). Elevated testosterone levels
  • Müllerian agenesis (MRKH): 46,XX phenotypically female. Ovaries present (normal estrogen + testosterone). Müllerian ducts fail to develop → no uterus, fallopian tubes, cervix, upper vagina. Primary amenorrhea with normal secondary sexual characteristics
  • Key differentiator: AIS = sparse pubic/axillary hair (androgens don't work). MRKH = normal pubic/axillary hair (ovaries produce androgens that work normally)
  • AIS management: Gonadectomy after puberty (intra-abdominal testes have malignant potential → gonadoblastoma/dysgerminoma). Estrogen replacement after gonadectomy
  • Müllerian duct derivatives: Fallopian tubes, uterus, cervix, upper 1/3 vagina (all absent in both AIS and MRKH)
AISTesticular FeminizationMüllerian AgenesisMRKHPrimary Amenorrhea46,XY FemaleAMH

AIS vs MRKH — Complete Comparison

FeatureAIS (Androgen Insensitivity)MRKH (Müllerian Agenesis)
Karyotype46,XY (genetically male)46,XX (genetically female)
GonadsTestes (intra-abdominal — no scrotal descent)Ovaries (normal, functional)
UterusAbsent (AMH from testes destroys Müllerian ducts)Absent (Müllerian ducts failed to develop)
Fallopian tubesAbsentAbsent
VaginaBlind-ending vaginal pouch or absentBlind-ending vaginal pouch or absent
Breast developmentTanner 5 (testosterone aromatized to estrogen by testes)Tanner 5 (normal ovarian estrogen)
Pubic/axillary hairTanner 1-2 (androgens don't work — receptor defect)Tanner 4-5 (androgens work normally from ovaries)
Testosterone levelHigh (normal male range — receptor absent)Normal female range
FSH/LH↑ (testes do not suppress pituitary normally)Normal
Cancer riskGonadoblastoma/dysgerminoma in intra-abdominal testesNone from ovaries
ManagementGonadectomy after puberty (allow estrogen-driven puberty first); then HRTVaginal dilators (progressive dilation to create functional vagina); uterus transplant being studied
Fertility potentialNone (no uterus, no eggs)Gestational surrogacy possible (own eggs + donor uterus)
Wolffian vs Müllerian Duct Derivatives

Wolffian (mesonephric) ducts → require testosterone → develop into: seminal vesicles, vas deferens, epididymis, ejaculatory duct. In AIS: testosterone is made but receptor is defective → Wolffian structures do NOT develop.

Müllerian (paramesonephric) ducts → require absence of AMH → develop into: fallopian tubes, uterus, cervix, upper vagina. AMH (from testes) destroys Müllerian ducts in males. In AIS: testes produce AMH → Müllerian ducts are destroyed → no uterus/fallopian tubes/upper vagina.

In MRKH (46,XX): No AMH produced (no testes) but Müllerian ducts simply fail to form — idiopathic agenesis.

1 episode
Gynecologic Oncology
Cervical cancer is a comprehensive systems question: it tests virology (HPV E6/E7 and p53/Rb), public health (vaccination and screening intervals), pathology (CIN grading, pap smear interpretation), and clinical management (colposcopy indications, LEEP complications). Endometrial and ovarian cancer are tested mainly on risk factors, tumor markers, and staging principles.
EP467
The Clutch Cervical Cancer Podcast
  • Presentation: Postcoital bleeding (most common symptom), pelvic pain, watery discharge. Most common cause of death = renal failure (ureter obstruction → obstructive uropathy → hydronephrosis)
  • HPV pathogenesis: High-risk HPV 16, 18 (and 31, 33). E6 protein = ubiquitin ligase for p53; E7 protein = ubiquitin ligase for Rb. Both tumor suppressors degraded → unregulated cell growth
  • Screening: Start at age 21 (regardless of sexual debut). Ages 21–29: Pap smear every 3 years. Ages 30–65: Pap smear every 3 years OR co-test (Pap + HPV) every 5 years. HIV patients: 2× in first year, then annually forever
  • 5 indications for colposcopy: HSIL, atypical glandular cells, ASC-H, positive high-risk HPV (HPV 16/18), repeated positive ASC-US. If abnormal pap but none of these 5 → test for high-risk HPV
  • CIN grading: CIN1 = lower 1/3 dysplasia. CIN2 = lower 2/3. CIN3 (HSIL) = full thickness → same as carcinoma in situ (cells have NOT broken basement membrane)
  • LEEP / conization risk: Both increase risk of cervical incompetence → painless second-trimester pregnancy loss (fetal descent through incompetent cervix). Treat with cervical cerclage
Cervical CancerHPVE6 E7Pap SmearColposcopyCINLEEPHSILDES

HPV Pathogenesis — Molecular Mechanism

E6 protein: Ubiquitin ligase that polyubiquitinates p53 → targets p53 to proteasome for degradation → loss of tumor suppressor → uncontrolled cell cycle progression past G1.

E7 protein: Ubiquitin ligase that polyubiquitinates Rb (retinoblastoma protein) → Rb degradation → E2F transcription factor released → unrestrained S-phase entry → uncontrolled DNA synthesis.

Both p53 and Rb are tumor suppressor genes. HPV's one strategy (E6 + E7) eliminates both checkpoints simultaneously — explaining its potent oncogenic potential.

Cervical Cancer Screening Algorithm

Age GroupScreening TestIntervalNotes
<21 yearsNoneDo not screen regardless of sexual debut
21–29 yearsPap smear aloneEvery 3 yearsNo HPV co-testing (too many transient HPV infections in this age group)
30–65 yearsPap smear alone OR Pap + HPV co-testEvery 3 years (Pap alone) or every 5 years (co-test)Co-test is preferred; negative co-test allows 5-year interval
>65 yearsStop if adequate prior screening + low riskContinue if: HIV, immunocompromised, DES exposure, prior CIN2+ history
HIV / immunocompromisedPap smearTwice in first year after HIV diagnosis → annually foreverNo stopping age for HIV patients
After hysterectomyStop (for benign indication)Continue if hysterectomy for malignant/premalignant lesion → annual vaginal cuff Pap

Pap Smear Result → Management

Pap ResultNext StepWhy
HSIL (CIN3)ColposcopyFull-thickness dysplasia — high risk of invasion; cannot wait
Atypical glandular cells (AGC)Colposcopy + endocervical curettage + endometrial biopsyMay indicate endocervical or endometrial malignancy — must assess entire reproductive tract
ASC-H (cannot exclude HSIL)ColposcopySuspicious for high-grade lesion
High-risk HPV 16 or 18 positiveColposcopyHighest-risk HPV types; immediate colposcopy regardless of cytology result
ASC-US (repeated positive)ColposcopyMultiple indeterminate results → pattern is enough to warrant direct visualization
ASC-US (single, first time)High-risk HPV reflex testingHPV testing trumps "repeat Pap in 6-12 months" if both options offered
LSIL (CIN1-2)High-risk HPV testing or repeat Pap in 12 monthsLow-grade lesions often regress spontaneously; monitor first
Atypical Glandular Cells (AGC) — Three-Part Workup

AGC is the one pap result that requires evaluating the ENTIRE reproductive tract (not just the cervix), because it can indicate pathology anywhere from cervix to uterine corpus:
1. Colposcopy — ectocervix
2. Endocervical curettage (ECC) — endocervical canal
3. Endometrial biopsy (endometrial sampling) — uterine cavity
All three must be done. Missing any one is an incomplete workup.

CIN Grading — Histological Classification

GradeUSMLE SynonymWhat It MeansBasement Membrane
CIN1LSIL (part of)Dysplasia in lower 1/3 of cervical epitheliumIntact
CIN2LSIL (part of)Dysplasia in lower 2/3 of cervical epitheliumIntact
CIN3HSIL = Carcinoma in SituDysplasia of FULL epithelial thickness; mitoses, nuclear atypia throughoutIntact (not yet invasive)
Invasive carcinomaCervical carcinomaCells break through basement membrane into stromaBreached

Treatment Options for CIN

ProcedureIndicationKey Complication
LEEP (Loop Electrosurgical Excision Procedure)CIN2-3; allows histology of specimenCervical incompetence → painless second-trimester loss; treat with cerclage in next pregnancy
Conization (cold-knife cone biopsy)HSIL extending into endocervical canal; same risk as LEEPCervical incompetence; same risk
CryotherapyCIN1-2 (older approach)Destroys biopsy specimen — cannot do histologic analysis; generally avoided
HysterectomyInvasive cervical cancerDefinitive for early stage; add radiation ± chemo for advanced
HPV Vaccination — Primary Prevention

Recommended ages: 9–26 years (routine at 11-12). Can extend to age 45 with shared decision-making.
Number of doses: 2 doses if starting before age 15; 3 doses if starting at 15 or older or immunocompromised.
Covers: HPV 6, 11 (condyloma), 16, 18, 31, 33, 45, 52, 58 (9-valent Gardasil).
Primary vs secondary prevention: Vaccination = primary (prevents HPV infection). Pap smear = secondary (detects precancerous lesions before invasive cancer).

Other Gynecologic Malignancies — High-Yield Summary

CancerMost Common TypeKey Risk FactorsTumor MarkerScreening
Endometrial cancerEndometrioid adenocarcinoma (type 1)Unopposed estrogen (obesity, PCOS, tamoxifen, exogenous estrogen without progestin, late menopause, nulliparity)CA-125 (less specific)No routine screening; biopsy triggered by abnormal uterine bleeding (AUB), especially postmenopausal
Ovarian cancerSerous cystadenocarcinoma (epithelial)BRCA1/2 mutation, family history, nulliparity, late menopause. OCP use is PROTECTIVE (↓ ovulations)CA-125 (monitoring, not screening); HE4No routine population screening; TVUS + CA-125 in high-risk (BRCA1/2)
Cervical cancerSquamous cell carcinoma (80%)HPV 16/18, multiple sexual partners, early coitus, immunosuppression, DES exposure (clear cell type)SCC antigenPap smear ± HPV (see above)
Vaginal cancerSquamous cell carcinoma (primary); clear cell adenocarcinoma (DES exposure)DES exposure → clear cell adenocarcinoma in young women (mother took DES during pregnancy)None specificNone routine
Vulvar cancerSquamous cell carcinomaHPV (younger women), lichen sclerosus (older women)None specificNone routine; biopsy persistent lesions
Gestational trophoblastic diseaseHydatidiform mole (complete > partial)Extremes of age, prior mole, Asian ethnicityβ-hCG (markedly elevated; complete mole β-hCG >100,000)Serial β-hCG after evacuation; methotrexate if persistent elevation (GTN)
Molar Pregnancy (GTD) — Key Numbers

Complete mole: 46,XX (all paternal — 2 sperm + empty egg); no fetal parts; β-hCG markedly elevated; "snowstorm" appearance on ultrasound; theca-lutein cysts (from excess β-hCG); 2% risk of choriocarcinoma.
Partial mole: Triploid (69,XXX or 69,XXY — 2 sperm + 1 egg); fetal parts present; lower β-hCG; less malignant potential.
Management: Suction curettage → weekly serial β-hCG until undetectable × 3 → avoid pregnancy for 6–12 months. Rising or plateauing β-hCG after evacuation = gestational trophoblastic neoplasia (GTN) → methotrexate.